Anti-neutrophil gelatinase-associated lipocalin (dog, NGAL)

Mouse monoclonal antibody, biotinylated

Cat.No. ABS 047-14B

Subclass Subclass IgG2b/k
Presentation:

Preparation: Biotinylated

Content: 50 µL, 1 mg/mL

Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide

Storage: In the dark at 4-8ºC

Antigen:

Dog neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically from renal tubular cells in response to ischemic or nephrotoxic injury.

Immunogen:

Recombinant dog NGAL adsorbed onto aluminum hydroxide gel

Specificity:

ABS 047-14 binds dog NGAL

Epitope specificity:

Not determined

Reactivity:

ABS 047-14 binds free dog NGAL in solution.

ABS 047-14 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 047-17 (as capture antibody) for measuring dog NGAL, giving a detection limit of 5 pg/mL in an unoptimized buffer assay.

Application:
Method Usability Dilution guideline References
ELISA Yes 1/10,000
Immunoblotting
Immunohistochemistry

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