Anti-neutrophil gelatinase-associated lipocalin (monkey, human, NGAL)
Mouse monoclonal antibody
Cat.No. ABS 038-23
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4,containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). Expression of NGAL in the kidney is dramatically increased by acute kidney injury (3).
Recombinant human NGAL adsorbed onto aluminum hydroxide gel
ABS 038-23 binds cynomolgus monkey (Macaca fascicularis) NGAL and cross-reacts with human NGAL.
ABS 038-23 binds human NGAL in complex with MMP-9
Differs from that of ABS 038-04,-15,-23,-26 and HYB 211-01,-02,-05
ABS 038-23 (as capture antibody) forms a sandwich pair with HYB 211-02 (as biotinylated detection antibody) for measuring cynomolgus monkey (Macaca fascicularis) and human NGAL.
ABS 038-23 binds human NGAL in solution.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/250 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Nor determined |
1. Kjeldsen L, Johnsen AH, Sengeløv H, Borregaard N (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem 268:10425-10432.
2. Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M, Kjeldsen L (1996) Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel diseases. Gut 38:414-420.
3. Matthaeus T, Schulze-Lohoff E, Ichimura T, Weber M, Andreucci M, Park KM, Alessandrini A, Bonventre JV (2001) Co-regulation of neutrophil gelatinase-associated lipocalin and matrixmetalloproteinase-9 in the postischemic rat kidney. J Am Soc Nephrol 12:787A.
