Research / ABS 038-14

Anti-neutrophil gelatinase-associated lipocalin (human, NGAL)

Cat.No. ABS 038-14

Class Mouse Monoclonal antibody
Subclass IgG1/k
Presentation: Preparation: Protein-A/G purified
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Antigen: Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). Expression of NGAL in the kidney is dramatically increased by acute kidney injury (3).
Immunogen: Recombinant human NGAL adsorbed onto aluminum hydroxide gel
Specificity: ABS 038-14 binds human NGAL and shows some cross-reactivity with NGAL from pig and dog
Epitope specificity: Differs from those of HYB 211-01, -02, ABS 038-04, -15, -26 and the ABS 062 series of antibodies
Reactivity: ABS 038-14 binds human NGAL in solution and coated onto a solid phase. ABS 038-14 (as capture antibody) forms sandwich pairs with HYB 211-01, HYB 211-02, ABS 038-04, ABS 038-15 and ABS 038-26 (as biotinylated detection antibodies) for measuring human NGAL.With ABS 038-15B detection antibody, the assay reacts equally with NGAL monomer and homodimer on a mass basis. With ABS 038-14B detection antibody, the assay is specific for NGAL homodimer and can be used at sample dilutions giving low levels of total NGAL.
Application:
Method Usability References
ELISA Yes -
Immunoblotting Yes -
Immunohistochemistry Not determined -

References:

1. Kjeldsen L, Johnsen AH, Sengeløv H, Borregaard N (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem 268:10425-10432.
2. Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M, Kjeldsen L (1996) Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel diseases. Gut 38:414-420.
3. Matthaeus T, Schulze-Lohoff E, Ichimura T, Weber M, Andreucci M, Park KM, Alessandrini A, Bonventre JV (2001) Co-regulation of neutrophil gelatinase-associated lipocalin and matrixmetalloproteinase-9 in the postischemic rat kidney. J Am Soc Nephrol 12:787A.