Research / ABS 062-14B

Anti-neutrophil gelatinase-associated lipocalin (human, NGAL)

Cat.No. ABS 062-14B

Class Mouse Monoclonal antibody, Biotinylated
Subclass IgG2a/k
Presentation: Preparation: Biotinylated
Content: 50 μL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Antigen: Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). Expression of NGAL in the kidney is dramatically increased by acute kidney injury (3).
Immunogen: Human recombinant NGAL coupled to carrier and adsorbed to aluminum hydroxide gel.
Specificity: ABS 062-14 is specific for human NGAL
Epitope specificity: Differs from those of HYB 211-01, the ABS 038 series of antibodies and ABS 062-33.
Reactivity: ABS 062-14 binds human NGAL coated onto a solid phase. ABS 062-14 forms a sandwich pair with HYB 211-01, ABS 038-04, -14, -15, -23, -26 and ABS 062-33 for measuring human NGAL. When ABS 062-14 is used as biotinylated detection antibody with ABS 062-33 as capture antibody, the assay is specific for NGAL monomer, cross-reacting <1% with NGAL homodimer.
Method Usability References
Immunoblotting Not determined
Immunohistochemistry Not determined