|Class||Mouse Monoclonal antibody|
|Presentation:||Preparation: Protein-A/G purified
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
|Antigen:||Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). Expression of NGAL in the kidney is dramatically increased by acute kidney injury (3).|
|Immunogen:||Human recombinant NGAL in monomer and dimer forms adsorbed to aluminum hydroxide gel|
|Specificity:||HYB 211-01 is specific for human NGAL. No cross-reaction with NGAL from other species has been detected.|
|Epitope specificity:||Differs from those of HYB 211-02 and the ABS 038 and ABS 062 series of antibodies.|
|Reactivity:||HYB 211-01 reacts strongly with human NGAL. Strong reaction is seen when used as detection antibody in sandwich ELISA in combination with a polyclonal NGAL capture antibody and when tested on recombinant NGAL coated directly onto the well. It can also be sued as capture antibody in sandwich ELISA with biotinylated HYB 211-02 as detection antibody. HYB 211-01 reacts with NGAL in immunochemical staining of paraformaldehyde-fixed sections. In Western blotting of SDS-PAGE gels, HYB 211-01 reacts with NGAL in both reduced and unreduced forms (4, 5)|
1. Kjeldsen L, Johnsen AH, Sengeløv H, Borregaard N (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem 268:10425-10432.
2. Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M, Kjeldsen L (1996) Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel diseases. Gut 38:414-420.
3. Matthaeus T, Schulze-Lohoff E, Ichimura T, Weber M, Andreucci M, Park KM, Alessandrini A, Bonventre JV (2001) Co-regulation of neutrophil gelatinase-associated lipocalin and matrixmetalloproteinase-9 in the postischemic rat kidney. J Am Soc Nephrol 12:787A.
4. Kjeldsen L, Koch C, Arnljots K, Borregaard N (1996) Characterization of two ELISAs for NGAL, a newly described lipocalin in human neutrophils. J Immunol Methods 198:155-164.
5. Hu L, Hittelman W, Lu T, Ji P, Arlinghaus R, Shmulevich I, Hamilton SR, Zhang W (2009) NGAL decreases E-cadherin-medicated cell-cell adhesion and increases cell motility and invasion through Rac1 in colon carcinoma cells. Lab Inbest 89:531-548.