Research / HYB 211-02B

Anti-Neutrophil gelatinase-associated lipocalin (human, NGAL)

Cat.No. HYB 211-02B

Class Mouse Monoclonal antibody, Biotinylated
Subclass IgG1/k
Presentation: Preparation: Biotinylated
Content: 50 μL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Antigen: Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). Expression of NGAL in the kidney is dramatically increased by acute kidney injury (3).
Immunogen: Human recombinant NGAL in monomer and dimer forms adsorbed to aluminium hydroxide gel
Specificity: HYB 211-02 is specific for human NGAL, and cross-reacts with cynomolgus monkey (Macaca fascicularis) NGAL.
Epitope specificity: Differs from those of HYB 211-01, ABS 038-14, -15, -23 and ABS 062-33.
Reactivity: HYB 211-02 reacts strongly with human NGAL. Strong reaction is seen when used as detection antibody in sandwich ELISA in combination with a polyclonal NGAL capture antibody and when tested on recombinant NGAL coated directly onto the well. HYB 211-02 reacts with NGAL in immunochemical staining of paraformaldehyde-fixed sections containing neutrophils. In Western blotting of SDS-PAGE gels, HYB 211-02 reacts with NGAL in both reduced and unreduced forms. A dilution guideline of 1/50 has proved successful (4).
HYB 211-02 (as biotinylated detection antibody) forms a sandwich pair with ABS 038-23 (as capture antibody) for measuring cynomolgus monkey (Macaca fascicularis) and human NGAL.
Method Usability References
Immunoblotting Not determined -
Immunohistochemistry Not determined -


1. Kjeldsen L, Johnsen AH, Sengeløv H, Borregaard N (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem 268:10425-10432.
2. Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M, Kjeldsen L (1996) Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel diseases. Gut 38:414-420.
3. Matthaeus T, Schulze-Lohoff E, Ichimura T, Weber M, Andreucci M, Park KM, Alessandrini A, Bonventre JV (2001) Co-regulation of neutrophil gelatinase-associated lipocalin and matrixmetalloproteinase-9 in the postischemic rat kidney. J Am Soc Nephrol 12:787A.
4. Kjeldsen L, Koch C, Arnljots K, Borregaard N (1996) Characterization of two ELISAs for NGAL, a newly described lipocalin in human neutrophils. J Immunol Methods 198:155-164.