Research / RUO Chemistry Reagents

The NGAL Test™ Reagent Kit - RUO

Cat.No. ST001RA

Application

The NGAL Test™ is a particle-enhanced turbidimetric immunoassay for the quantitative determination of NGAL in human urine, EDTA plasma or heparin plasma.

For research use only. Not for use in diagnostic procedures.


Assay specifications

Method Particle-enhanced turbidimetric immunoassay (PETIA)
Sample Urine, heparin and EDTA plasma
Number of tests Approx. 100 (Can vary based on analyzer model)
Assay time Approx. 10 minutes
Measuring range 25 to 5,000 ng/mL
Shelf life 24 months from manufacturing. See expiry date on the label
Stability after opening 4 weeks at 2-8°C. On the Hitachi 917 analyzer, the manufacturer obtains satisfactory performance for up to 8 weeks.
On board stability 4 weeks at correct temperature (2-8°C) in appropriate containers. On the Hitachi 917 analyzer, the manufacturer obtains satisfactory performance for up to 8 weeks on board.
Sample stability If testing after 24 hours of sampling, freeze samples at -20°C or below
For long-term storage of specimens, -70°C or below is recommended
Application RUO - Research Use Only.  Not for use in diagnostic procedures


Assay procedure

The assay should be carried out in accordance with the specific application note for the chemistry analyzer to be used.


What is NGAL?

NGAL (neutrophil gelatinase-associated lipocalin, lipocalin-2, siderocalin) is a small protein expressed in neutrophils and certain epithelia, including the renal tubules. Renal expression of NGAL is dramatically increased in kidney injury from a variety of causes, and NGAL is released into both urine and plasma.