|Target||Dog NGAL (Neutrophil Gelatinase-Associated Lipocalin|
|Sample Type||Dog urine, plasma or serum, tissue extracts or culture media|
|Numbers of tests||96 wells - 40 test in duplicate|
|Sample vol needed||10 µl|
|Assay range||4-400 pg/mL|
|Limit of Detection||0.56 pg/mL|
|Assay time||<4 hours|
|Result type||Quantitative (pg/mL)|
|Application||For Research Use Only (RUO)|
With the Dog NGAL ELISA Kit, urine, plasma or serum samples can be analyzed in a standard laboratory with conventional ELISA equipment. All components are stored at 4°C and all incubations are performed at room temperature. The kit contains ready-to-use reagents and a pre-coated 96-well ELISA plate, which greatly reduces the hands-on time. The simple test protocol permits good reproducibility with a total assay time under 4 hours.
Dogs provide additional information in the study of kidney function, surgical procedures and treatment in relation to human disease, and canine studies continue to be important in drug development. The dog NGAL ELISA test is non-invasive, and gives an early indication of kidney damage, uses little sample and is easy to perform. NGAL measurement in dogs may prove to be of considerable value in the preclinical evaluation of new pharmaceutical products, as it is much cheaper and easier to perform than microscopic examination of the kidney, which has hitherto been the usual procedure.
The test can potentially be used to measure drug efficacy in the development of new treatments for kidney damage, to assess safety or nephrotoxic side effects of new drugs, and can be used in basic research on kidney injury and kidney diseases, e.g. in the folowing settings: