Research / Pig NGAL ELISA

Pig NGAL ELISA Kit

Cat.No. KIT 044

An ELISA for the in vitro determination of pig NGAL in urine, plasma, serum, tissue extracts or culture media.
 
Target Pig NGAL (Neutrophil Gelatinase-Associated Lipocalin
Sample Type Pig urine, plasma or serum, tissue extracts or culture media
Cross reaction None
Numbers of tests 96 wells - 40 test in duplicate
Sample vol needed 10 µl
Dilution guideline NA
Assay range 4-400 pg/mL
Limit of Detection 1 pg/mL
Assay time <4 hours
Result type Quantitative (pg/mL)
Application For Research Use Only (RUO)
 

Pig NGAL - a biomarker for nephrotoxicity in drug development

With the new Pig NGAL ELISA Kit, urine, plasma or serum samples can be analyzed in a standard laboratory with conventional ELISA equipment. All components are stored at 4°C and all incubations are performed at room temperature. The kit contains ready-to-use reagents and a pre-coated 96-well ELISA plate, which greatly reduces the hands-on time. The simple test protocol permits good reproducibility with a total assay time under 4 hours.

Kit features

  • Highly reproducible and easy to use
    • Ready-to-use working solutions
    • Ready-to-use calibrators
    • Pre-coated 96-well ELISA plate
    • All incubations performed at room temperature
  • Easy storage
    • All components are stored at 4°C
  • Requires only standard ELISA equipment

Applicability

The pig is an often used experimental model for medical research because of its close resemblance to humans. Pigs are being used in everything from experimental treatments to experimental surgical procedures. The pharmaceutical industry is also looking more and more to pigs as a model for drug safety assessment in drug development as pigs are claimed to be more robust than the traditional dog models and in some cases a better mirror model of humans than dogs or even monkeys.

The Pig NGAL ELISA Kit allows for the determination of rises in pig NGAL concentrations and hence the extent of renal injury, including nephrotoxic injury, to be determined, e.g. in the following settings:

  • Medical research
  • Veterinary research
  • Discovery, Research and Development
  • Pre-clinical phase