Research / rec Human NGAL

recombinant Human NGAL protein

Cat.No. SP001RA

Presentation: Production:E. coli
Source: Full length amino acid sequence
Content: 1 mL, 1000 pg/mL +/-15%
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl, 0.5% BSA; 0.5% Tween 20; 0.0016% Phenol red; 0.1% Proclin 950
Storage: 2-8ºC
Antigen: Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (2). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (3). Expression of NGAL in the kidney is dramatically increased by acute kidney injury

This product is purified full length recombinant protein without any tags, produced from E. coli

Application:
Method Usability References
ELISA Yes -
Immunoblotting ND -
Immunohistochemistry ND -

General references:
1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A
2. Kjeldsen L et al (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem 268:10425-10432.
3. Nielsen BS et al (1996) Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel diseases. Gut 38:414-420