Research / rec Rat NGAL

recombinant Rat NGAL protein

Cat.No. SP003RA

Presentation: Production: E. coli
Source: Full length amino acid sequence
Content: 1 mL, 400 pg/mL +/- 15%
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl, 0.5% BSA; 0.5% Tween 20; 0.0016% Phenol red; 0.1% Proclin 950
Storage: 2-8ºC

This product is purified full length recombinant protein without any tags, produced from E. coli

Antigen:

Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin-2, 25-kDa alpha 2-microglubin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including cells tubular cells of the kidney. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is up-regulated in a variety of pathological situations including infection, inflammation, renal injury and certain adenocarcinomas.

Application:
Method Usability References
ELISA Yes -
Immunoblotting ND -
Immunohistochemistry ND -

Generel References:
1. Mishra J et al (2003) Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14:2534-43.
2. Zhang J et al (2009) NGAL Expression in Kidney of Spontaneously Hypertensive Rats (SHR) Treated with Doxorubicin (DXR) Alone or Combined with Dexrazoxane (DRZ) (Presented at Tox Expo 2009)
3. Adler M et al (2010) Assessment of candidate biomarkers of drug-induced hepatobiliary injury in preclinical toxicity studies. Toxicology Letters 196:1-11.